Use rolling-ball algorithm for estimating background intensity¶
The rolling-ball algorithm estimates the background intensity of a grayscale image in case of uneven exposure. It is frequently used in biomedical image processing and was first proposed by Stanley R. Sternberg in 1983 1.
The algorithm works as a filter and is quite intuitive. We think of the image as a surface that has unit-sized blocks stacked on top of each other in place of each pixel. The number of blocks, and hence surface height, is determined by the intensity of the pixel. To get the intensity of the background at a desired (pixel) position, we imagine submerging a ball under the surface at the desired position. Once it is completely covered by the blocks, the apex of the ball determines the intensity of the background at that position. We can then roll this ball around below the surface to get the background values for the entire image.
Scikit-image implements a general version of this rolling-ball algorithm, which allows you to not just use balls, but arbitrary shapes as kernel and works on n-dimensional ndimages. This allows you to directly filter RGB images or filter image stacks along any (or all) spacial dimensions.
Sternberg, Stanley R. “Biomedical image processing.” Computer 1 (1983): 22-34. DOI:10.1109/MC.1983.1654163
Classic rolling ball¶
In scikit-image, the rolling ball algorithm assumes that your background has low intensity (black), whereas the features have high intensity (white). If this is the case for your image, you can directly use the filter like so:
import imageio import matplotlib.pyplot as plt import numpy as np from skimage import ( data, restoration, util ) def plot_result(image, background): fig, ax = plt.subplots(nrows=1, ncols=3) ax.imshow(image, cmap='gray') ax.set_title('Original image') ax.axis('off') ax.imshow(background, cmap='gray') ax.set_title('Background') ax.axis('off') ax.imshow(image - background, cmap='gray') ax.set_title('Result') ax.axis('off') fig.tight_layout() image = data.coins() background = restoration.rolling_ball(image) plot_result(image, background) plt.show()
If you have dark features on a bright background, you need to invert the image before you pass it into the algorithm, and then invert the result. This can be accomplished via:
image = data.page() image_inverted = util.invert(image) background_inverted = restoration.rolling_ball(image_inverted, radius=45) filtered_image_inverted = image_inverted - background_inverted filtered_image = util.invert(filtered_image_inverted) background = util.invert(background_inverted) fig, ax = plt.subplots(nrows=1, ncols=3) ax.imshow(image, cmap='gray') ax.set_title('Original image') ax.axis('off') ax.imshow(background, cmap='gray') ax.set_title('Background') ax.axis('off') ax.imshow(filtered_image, cmap='gray') ax.set_title('Result') ax.axis('off') fig.tight_layout() plt.show()
Be careful not to fall victim to an integer underflow when subtracting a bright background. For example, this code looks correct, but may suffer from an underflow leading to unwanted artifacts. You can see this in the top right corner of the visualization.
image = data.page() image_inverted = util.invert(image) background_inverted = restoration.rolling_ball(image_inverted, radius=45) background = util.invert(background_inverted) underflow_image = image - background # integer underflow occurs here # correct subtraction correct_image = util.invert(image_inverted - background_inverted) fig, ax = plt.subplots(nrows=1, ncols=2) ax.imshow(underflow_image, cmap='gray') ax.set_title('Background Removal with Underflow') ax.axis('off') ax.imshow(correct_image, cmap='gray') ax.set_title('Correct Background Removal') ax.axis('off') fig.tight_layout() plt.show()
rolling_ball can handle datatypes other than np.uint8. You can
pass them into the function in the same way.
However, you need to be careful if you use floating point images
that have been normalized to
[0, 1]. In this case the ball will
be much larger than the image intensity, which can lead to
radius=70.5 is much larger than the maximum intensity of
the image, the effective kernel size is reduced significantly, i.e.,
only a small cap (approximately
radius=10) of the ball is rolled
around in the image. You can find a reproduction of this strange
effect in the
Advanced Shapes section below.
To get the expected result, you need to reduce the intensity of the
kernel. This is done by specifying the kernel manually using the
Note: The radius is equal to the length of a semi-axis of an ellipsis, which is half a full axis. Hence, the kernel shape is multipled by two.
rolling_ball uses a ball shaped kernel (surprise).
Sometimes, this can be too limiting - as in the example above -,
because the intensity dimension has a different scale compared to
the spatial dimensions, or because the image dimensions may have
different meanings - one could be a stack counter in an image stack.
To account for this,
rolling_ball has a
which allows you to specify the kernel to be used. A kernel must
have the same dimensionality as the image (Note: dimensionality,
not shape). To help with it’s creation, two default kernels are
ball_kernel specifies a ball shaped
kernel and is used as the default kernel.
specifies an ellipsoid shaped kernel.
You can also use
ellipsoid_kernel to recreate the previous,
unexpected result and see that the effective (spatial) filter size
Another feature of
rolling_ball is that you can directly
apply it to higher dimensional images, e.g., a z-stack of images
obtained during confocal microscopy. The number of kernel
dimensions must match the image dimensions, hence the kernel shape
is now 3 dimensional.
A kernel size of 1 does not filter along this axis. In other words, above filter is applied to each image in the stack individually.
However, you can also filter along all 3 dimensions at the same time by specifying a value other than 1.
Another possibility is to filter individual pixels along the planar axis (z-stack axis).
Total running time of the script: ( 1 minutes 19.126 seconds)